Current Issue : October - December Volume : 2016 Issue Number : 4 Articles : 6 Articles
Doxorubicin is a commonly used anticancer agent, which may cause cardiac toxicity. The present\nstudy designed to evaluate Phoenix dactylofera (dates) in doxorubicin (DXR) induced cardiac toxicity\nand cardiac remodeling in Wistar albino rats. The experimental rats procured, acclimatized\nand finally divided into five groups (n = 6). Group I served as normal controls, group II served as\ndisease controls and groups 3, 4 & 5 served as therapeutic groups (Phoenix dactylofera 5%, 10%,\nand 15% respectively). Cardiac remodeling and toxicity in the rats were induced by administration\nof DXR (1.25 mg/kg i.p. in 16 divided doses/month). At the end of protocol, effect of Phoenix\ndactylofera on cardiac remodeling was evaluated by measuring parameters like haemodynamics,\nheart weight, anatomy, Troponin T, creatine phosphokinase (CPK), creatine phosphokinase-MB\n(CPK-MB), Lactate dehydrogenase (LDH), serum glutamate oxaloacetate transaminase (SGOT),\nserum glutamate pyruvate transaminase (SGPT), calcium ion Ca2+, sodium ion Na+, potassium ion\nK+, intracellular enzymes like Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase\n(SOD) and catalase (CAT). The disease control groups showed significantly elevated (p < 0.001)\nlevels of troponin T, CPK, CPK-MB, LDH, MDA, and significantly reduced levels (p < 0.001) of GSH,\nSOD & CAT while the levels of SGPT, SGOT were increased less significantly (p < 0.01) as compared\nto therapeutic groups. Treatment with Phoenix dactylofera significantly (p < 0.01) reduced the increased\nlevels of Troponin T, CPK, CPK-MB, LDH, SGOT, SGPT, MDA, GSH, SOD & CAT as well as restored\nCa2+, Na+, K+ levels to a normal value. Further, the histological studies of the cardiac tissues\ndemonstrated that the normal architecture of the cardiac cells was restored in the animals fed with dietary Phoenix dactylofera as compared to disease controls. The findings show that the administration\nof Phoenix dactylofera has the potential to prevent the toxicity induced by doxorubicin\nin the experimental rats....
Background. Bacterial sepsis is amajor cause of illness in human immunodeficiency virus infected patients. There is scarce evidence\nabout sepsis among HIV patients in Ethiopia. This study aimed to determine the etiologic agents of bacterial sepsis and their\nantibiotic susceptibility patterns among HIV infected patients. Methods. A cross-sectional study was carried out from March 1\nto May 2, 2013. One hundred patients infected with HIV and suspected of having sepsis were included. Sociodemographic data\nwere collected by interview and blood sample was aseptically collected from study participants. All blood cultures were incubated\naerobically at 35âË?Ë?C and inspected daily for 7 days.Thepositive blood cultures were identified following the standard procedures and\nantimicrobial susceptibility testing was performed using disk diffusion technique. Data was entered by Epi-info version 3.5.1 and\nanalysis was done using SPSS version 20. Results. Of the study participants, 31 (31%) confirmed bacterial sepsis. The major isolates\nwere 13 (13%) Staphylococcus aureus, 8 (8%) coagulates negative staphylococci, and 3 (3%) viridans streptococci. Majority of the\nisolates, 25 (80.6%), were multidrug resistant to two or more antimicrobial agents. Conclusions. Bacterial sepsis was a major cause\nof admission for HIV infected patients predominated by Staphylococcus aureus and coagulase negative staphylococci species and\nmost of the isolates were multidrug resistant....
Our aim was to study the selected cases of the patients with ischemic heart disease\nand to analyze the structure of blood serum of patients in comparison with control\nserum of healthy subjects by methods: synchronous fluorescence fingerprint and\natomic force microscopy that are still not used in clinical practice. Our results of fluorescence\nanalysis showed that blood serum of all patients with ischemic heart disease\ndecreased intensity of fluorescence in comparison with control blood serum.\nEndogenous fluorescence of synchronous fluorescence fingerprint of blood serum of\npatients with unstable angina pectoris state after non ST elevation myocardial infarction;\nangina pectoris and arterial hypertension 3 was similar, but atomic force microscopy\nrevealed differences in the structure of blood serum of patients with the angina\npectoris. Blood serum of patients with angina pectoris exhibited disappearance\nof fluorescence peak with maximum fluorescence and showed lower fluorescence intensity\nthan control blood serum and blood serum of patients with arterial hypertension\n2. Profiles of synchronous fluorescence fingerprint of blood serum of patients\nwith arterial hypertension stage 2 showed formation of the new fluorescent peak with\nmaximum fluorescence, similar shape of synchronous fluorescence fingerprint and\nhigher fluorescence intensity than blood serum of healthy subjects. Blood serum sensitively\nrevealed changes in the body by using untraditional novel techniques which\nenable the analysis of the mixture of blood serum and might be a new possibility in\nstudy of heart ischemia diseases....
Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet\nactivation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of\nPRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture\nof CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours\nand samples were evaluated for the release of VEGF, TGF-...
Background: Buprenorphine is a semi-synthetic opioid used for the treatment of opioid dependence. Opioid use,\nincluding buprenorphine, has been increasing in recent years, in the general population and in pregnant women.\nConsequently, there has been a rise in frequency of neonatal abstinence syndrome (NAS), associated with buprenorphine\nuse during pregnancy. The purpose of this study was to investigate correlations between buprenorphine and\nbuprenorphine-metabolite concentrations in cord blood and onset of NAS in buprenorphine exposed newborns.\nMethods: Nineteen (19) newborns who met inclusion criteria were followed after birth until discharge in a doubleblind\nnon-intervention study, after maternal consent. Cord blood and tissue samples were collected and analyzed by\nliquid chromatographyââ?¬â??mass spectrometry (LCââ?¬â??MS) for buprenorphine and metabolites. Simple and multiple logistic\nregressions were used to examine relationships between buprenorphine and buprenorphine metabolite concentrations\nin cord blood and onset of NAS, need for morphine therapy, and length of stay.\nResults: Each increase in 5 ng/ml level of norbuprenorphine in cord blood increases odds of requiring treatment by\nmorphine 2.5 times. Each increase in 5 ng/ml of buprenorphine-glucuronide decreases odds of receiving morphine\nby 57.7 %. Along with concentration of buprenorphine metabolites, birth weight and gestational age also play important\nroles, but not maternal buprenorphine dose.\nConclusions: LCââ?¬â??MS analysis of cord blood concentrations of buprenorphine and metabolites is an effective way to\nexamine drug and metabolite levels in the infant at birth. Cord blood concentrations of the active norbuprenorphine\nmetabolite and the inactive buprenorphine-glucuronide metabolite show promise in predicting necessity of treatment\nof NAS. These finding have implications in improving patient care and reducing healthcare costs if confirmed in\na larger sample....
This research was aimed at discovering the serological and histological changes in cardiac and hepatic tissue after electric shock.\nThe CK-MB, ALT, and AMS indexes were tested with serological methods.Moreover, the Bcl-2, Bax, and Hsp-60 expression levels\nwere carefully measured. An electrical injury model was established by giving rats electric shocks at 110V with alternating electric\ncurrent. Blood samples from the rats were analyzed for the biochemical indexes. The degrees of pathological changes in the heart\nand liver were evaluated using IHC staining for Bcl-2, Bax, and Hsp-60. The levels of CK-MB in the electrical injury group rapidly\npeaked at 0.5 hours after the electric shock. Additionally, the levels of Bcl-2, Bax, and Hsp-60 in the cardiac and hepatic tissues\nchanged regularly after the electrical injury and exhibited apparent differences from the levels in the control group. CK-MB, ALT,\nand AMS were altered regularly after electric shock, and these results provide significant information for clinical and medicolegal\npractice. This research has shed light on the assessment of electrical injury without obvious electrical burns. Furthermore, the\nfindings obtained for Bcl-2/Bax and Hsp-60 can also facilitate pathological diagnosis and the identification of antemortem and\npostmortem electrical injury....
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